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The profile of cross-reaction with wheat germ agglutinin, used here as a general carbohydrate reagent, is shown in vacuum blots.
Larger cDNAs fragments of two of the SSH clones have been used as probes on northern blots and shown to be upregulated during infection.
Strong hybridisation signals were observed for all symptomatic samples, when blots were washed at medium to high stringency.
These blots are her handiwork.
All DNA used in Southern blots was also digested with 0.25 µg Ribonuclease A per µg DNA upon completion of restriction endonuclease digestion.