Frozen cell pellets were thawed on ice over night and re-suspended in a total volume of 50 ml lysis buffer.
When such holes freeze, and a rain succeeds, and finally a new freezing forms a fresh smooth ice over all, it is beautifully mottled internally by dark figures, shaped somewhat like a spider's web, what you may call ice rosettes, produced by the channels worn by the water flowing from all sides to a centre.
Fortunately, the rivers do not ice over until January, allowing for holiday travel through December.
