Albany had to blockade Margaret in Stirling Castle before she would surrender her sons, After being obliged to capitulate, Margaret returned to Edinburgh, and being no longer responsible for the custody of the king she fled to England in September, where a month later she bore to Angus a daughter, Margaret, who afterwards became countess of Lennox, mother of Lord Darnley and grandmother of James I.
He married a great-granddaughter of Duncan, 8th earl of Levenax (or Lennox), and besides this relationship by marriage the Napiers claimed a lineal male cadency from the ancient family of Levenax.
The legend with regard to the origin of the name Napier was given by Sir Alexander Napier, eldest son of John Napier, in 1625, in these words: "One of the ancient earls of Lennox in Scotland had issue three sons: the eldest, that succeeded him to the earldom of Lennox; the second, whose name was Donald; and the third, named Gilchrist.
The then king of Scotland having wars, did convocate his lieges to battle, amongst whom that was commanded was the earl of Lennox, who, keeping his eldest son at home, sent his two sons to serve for him with the forces that were under his command...
On the demand of Lennox, Darnley's father, Bothwell was put upon his trial, in April, but Lennox, having been forbidden to enter the city with more than six attendants, refused to attend, and Bothwell was declared not guilty.