The MLS BER has the potential to improve the diagnostic value of conventional BER in babies who suffered birth asphyxia.
Once bound the resin was washed with a minimum of 15 mls binding buffer followed by a minimum of 20 mls wash buffer.
A general baby massage oil blend is 1 drop each lavender, mandarin and roman chamomile in 30 mls carrier oil.
Finally the protein was eluted with 15 MLS of eluted with 15 MLS of Elute buffer with 2 ml fractions collected into eppendorf tubes.
This provoked a miniature flurry of responses from both members and non-members of MLS.
